COLO-668

Frequently Asked Questions

• What is the origin of COLO-668 cell line?

  COLO-668 cells were derived from a metastatic site of a human colorectal adenocarcinoma. They are widely used in cancer research, particularly in studies related to colorectal cancer biology, drug testing, and molecular studies.

• What is the recommended seeding density?

  The recommended seeding density for COLO-668 cells can vary depending on the specific experimental requirements and growth characteristics of the cells. As a general guideline:

  Initial Seeding Density: COLO-668 cells are typically seeded at a density ranging from 1 × 10^5 to 5 × 10^5 cells per milliliter (cells/mL) of medium.

  Adherence Considerations: COLO-668 cells are epithelial in origin and generally adhere well to tissue culture vessels. However, certain conditions or issues may affect their adherence:

• Why are my COLO-668 cells not adhering to the culture vessel?

  Cell Health: Ensure that the cells are healthy and not undergoing stress due to factors like incorrect medium composition, pH imbalance, or excessive handling.

  Surface Coating: COLO-668 cells may require specific surface coatings (e.g., collagen, fibronectin) to promote adherence. Check if the culture vessel has been properly coated before seeding.

  Cell Density: If seeded at too low a density, cells might not adhere well. Try increasing the seeding density slightly or adjusting the incubation time after seeding to allow for attachment.

  Cell Passage Number: Higher passage number cells may have reduced adherence properties. Consider using cells from earlier passages if adherence remains an issue.

  Medium Composition: Ensure the medium is appropriate for COLO-668 cells and contains necessary supplements for their growth and adherence.

  By addressing these factors, you can optimize the conditions for COLO-668 cell culture and promote adherence to the culture vessel surface, ensuring robust growth and experimental success.